<p><strong>产品属性 Product Attributes </strong></p> <p><strong>基本信息</strong></p> 中文名称: 小鼠甲胎蛋白检测试剂盒 <br/>英文名称: Mouse AFP ELISA Kit <br/>别名: AFP; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP<br/>储存条件: 2-8℃,6 months<br/>规格: 48T ; 96T<br/>检测目标: AFP<br/>样品类型: 血清/血浆/细胞培养上清<br/>反应性: Mouse<br/>应用: ELISA<br/>反应类型: Sandwich-ELISA<br/>定量/定性: 定量检测<br/>检测范围: 62.5-4000 pg/ml<br/>灵敏度: 31.25 pg/ml<br/>特异性: 不与小鼠Leptin,Prolactin,Resistin,VitaminDBP,人AFP等反应。<br/>精密度: 板内,板间变异系数均<10%<br/>回收率: 80%-120%<br/>反应时间: 3h<p><strong>Elisa信息</strong></p> Swiss Prot: P02772<br/>Gene ID: 11576<br/>检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠AFP单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的小鼠AFP会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗小鼠AFP抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠AFP发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的小鼠AFP,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的小鼠AFP浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠AFP的浓度。<br/>背景说明: alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. <p><strong>产品介绍 Product Introduction</strong></p>
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