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AC18148

猪胰岛素样生长因子结合蛋白3检测试剂盒,BR

Porcine IGFBP-3 ELISA Kit(订货以英文名为准)
品牌 货号 规格 包装 库存 目录价 会员价 操作
Acmec AC18148-48T BR 48T
¥1600.00
Acmec AC18148-96T BR 96T
¥2560.00
<p><strong>产品属性 Product Attributes </strong></p> <p><strong>基本信息</strong></p> 中文名称: 猪胰岛素样生长因子结合蛋白3检测试剂盒 <br/>英文名称: Porcine IGFBP-3 ELISA Kit <br/>别名: binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; insulin-like growth factor-binding protein 3<br/>储存条件: 2-8℃,6 months<br/>规格: 48T ; 96T<br/>检测目标: IGFBP-3<br/>样品类型: 血清/血浆/细胞培养上清<br/>反应性: Porcine<br/>应用: ELISA<br/>反应类型: Sandwich-ELISA<br/>定量/定性: 定量检测<br/>检测范围: 125-8000 pg/ml<br/>灵敏度: 62.5 pg/ml<br/>特异性: 不与ApoA1,BMP1,BMP2,BMP3,BMP4,CCL4/MIP-1β,CRP,HSP27,IL-1β,IL-1ra,IL-2,IL-4,IL-5,IL-6,IL-8,IL-12,IL-15,IL-17C,IL-21,IL-23,IL2R,IL6R,IFN-γ,PDGF,PLA2G7,prolactin,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF等反应。<br/>精密度: 板内,板间变异系数均<10%<br/>回收率: 80%-120%<br/>反应时间: 2.33h<p><strong>Elisa信息</strong></p> Swiss Prot: P16611<br/>Gene ID: 448812<br/>检测原理: Acmec ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪IGFBP-3单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的猪IGFBP-3会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗猪IGFBP-3抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的猪IGFBP-3发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的猪IGFBP-3,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的猪IGFBP-3浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中猪IGFBP-3的浓度。<br/>背景说明: The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation. <p><strong>产品介绍 Product Introduction</strong></p> 暂无数据 <p><strong> 注意事项 Considerations</strong></p> 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。 <br/>2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。 <br/>3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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